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lympholyte poly solution  (Cedarlane)


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    Structured Review

    Cedarlane lympholyte poly solution
    Lympholyte Poly Solution, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lympholyte+poly+solution/Lympholyte%C2%AE-poly+Cell+Separation+Media+(Isolation+of+PMNs+from+Human+peripheral+blood)%2C+1%2E113+%C2%B1+0%2E001+g%2Fml+%4025%C2%B0C/pm32658599-283-8-11
    Average 93 stars, based on 34 article reviews
    lympholyte poly solution - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Isolation:

    Article Title: Cathelicidin-mediated lipopolysaccharide signaling via intracellular TLR4 in colonic epithelial cells evokes CXCL8 production.
    Article Snippet: HT29 cells were co-transfected with the construct (1 μg) and pRL (0.1 μg) in DMEM media (FBS and antibiotic free) using TransIT transfection reagent (MIR5405; Mirus Bio LLC). .. Human neutrophils from healthy donors were isolated using Lympholyte®-poly solution (CL5070; Cedarlane) and stimulated with supernatants (500 μL) harvested from HT29 cells, either untreated (control; serum-free DMEM media) or treated with LPS ± LL-37 (for 4 h). .. Recombinant human interleukin-1β (IL-1β; 10 ng/ mL, 8900; Cell Signaling Technology) was used as a positive control for CXCL8 promoter assay.

    Article Title: Cathelicidin senses enteric pathogen Salmonella typhimurium/ LPS for colonic chemokine generation: a new innate immune role for a host defense peptide
    Article Snippet: .. Neutrophils were isolated by Lympholyte ® -poly solution (CL5070; Cedarlane) density gradient centrifugation of heparin-anticoagulated blood, obtained according to ethics-approved procedures from healthy human male donors. ..

    Article Title: Cathelicidin-mediated lipopolysaccharide signaling via intracellular TLR4 in colonic epithelial cells evokes CXCL8 production
    Article Snippet: Integrated fluorescence intensity per mouse was calculated using ImageJ 1.50i software in five randomly selected fields of view and data were reported as MFI from n = 4 mice. .. Human neutrophils from healthy donors were isolated using Lympholyte®-poly solution (CL5070; Cedarlane) and stimulated with supernatants (500 μL) harvested from HT29 cells, either untreated (control; serum-free DMEM media) or treated with LPS ± LL-37 (for 4 h). .. Immunofluorescence analysis was performed as described above, using an anti-neutrophil elastase antibody (5 μg/mL; MAB91671; R&D Systems) and secondary Alexa549-conjugated donkey anti-mouse IgG antibody.

    Control:

    Article Title: Cathelicidin-mediated lipopolysaccharide signaling via intracellular TLR4 in colonic epithelial cells evokes CXCL8 production.
    Article Snippet: HT29 cells were co-transfected with the construct (1 μg) and pRL (0.1 μg) in DMEM media (FBS and antibiotic free) using TransIT transfection reagent (MIR5405; Mirus Bio LLC). .. Human neutrophils from healthy donors were isolated using Lympholyte®-poly solution (CL5070; Cedarlane) and stimulated with supernatants (500 μL) harvested from HT29 cells, either untreated (control; serum-free DMEM media) or treated with LPS ± LL-37 (for 4 h). .. Recombinant human interleukin-1β (IL-1β; 10 ng/ mL, 8900; Cell Signaling Technology) was used as a positive control for CXCL8 promoter assay.

    Article Title: Cathelicidin-mediated lipopolysaccharide signaling via intracellular TLR4 in colonic epithelial cells evokes CXCL8 production
    Article Snippet: Integrated fluorescence intensity per mouse was calculated using ImageJ 1.50i software in five randomly selected fields of view and data were reported as MFI from n = 4 mice. .. Human neutrophils from healthy donors were isolated using Lympholyte®-poly solution (CL5070; Cedarlane) and stimulated with supernatants (500 μL) harvested from HT29 cells, either untreated (control; serum-free DMEM media) or treated with LPS ± LL-37 (for 4 h). .. Immunofluorescence analysis was performed as described above, using an anti-neutrophil elastase antibody (5 μg/mL; MAB91671; R&D Systems) and secondary Alexa549-conjugated donkey anti-mouse IgG antibody.

    Gradient Centrifugation:

    Article Title: Cathelicidin senses enteric pathogen Salmonella typhimurium/ LPS for colonic chemokine generation: a new innate immune role for a host defense peptide
    Article Snippet: .. Neutrophils were isolated by Lympholyte ® -poly solution (CL5070; Cedarlane) density gradient centrifugation of heparin-anticoagulated blood, obtained according to ethics-approved procedures from healthy human male donors. ..



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